System and Methods for Generating Three-Dimensional Images from Two-Dimensional Bioluminescence Images and Visualizing Tumor Shapes and Locations

Setting up projection plane geometry for images taken from different views. (a) some example views for consecutive images. (b) all views forming a full circle.


Invention Summary:

Bioluminescence imaging (BLI) is an emerging technique for sensitive and non-invasive imaging, which can be used for monitoring molecular events in living animals. Important applications of this technique include gene therapy and cell trafficking studies. Recent research has focused on bioluminescence tomography (BLT) in an effort to extract depth information from 2D bioluminescence images. Such efforts, however, have been largely ineffective in generating useful 3D images with high resolution. As such, although BLI is a useful imaging modality for generating 2D images, currently no practical technique for rapidly generating three-dimensional (3D) images from a series of 2D BLI images exists.

Researchers at Rutgers have developed a system and methods for reconstructing 3D images from 2D bioluminescent images and visualizing tumor shapes and locations. A plurality of 2D bioluminescent images of the subject(s) is acquired using any suitable bioluminescent imaging system. The 2D images are taken during a complete revolution about an axis of the subject, so that the imaging system is rotated about the subject by a predetermined angle between each image. Optionally, the imaging system could be held in a statio nary position, the subject could be rotated through a complete revolution, and a plurality of images could be taken.

All of the 2D images are processed in the same manner, so that the resulting 3D image of a tumor is generated. The subject itself, as well as a container holding the subject, can also be rendered in the 3D image using the orthographic projection technique. The tumor can be registered with a reference image of the subject or an image of the container so that the precise shape and location of the tumor can be visualized, together with detailed anatomical structure information extracted from other imaging modalities such as microCT.

Market Application:

  • Generating 3D images from 2D bioluminescent images and visualizing tumor locations
  • Gene therapy and cell trafficking studies

Advantages:

  • Does not require using multiple CCD cameras
  • Container holding the subject can also be rendered in the 3D image
  • Tumor can be registered with a reference image of the subject or an image of the container so that the precise shape and location of the tumor can be visualized

Intellectual Property & Development Status:

Issued US Patent 8,218,836.

Rutgers ID: 2005-118
Category(s):
Life Sciences
Software
Diagnostics
Information Technology
Contact:
Andrea Dick
Senior Licensing Manager
848-932-4018
aid8@ored.rutgers.edu
Inventors:
Dimitris Metaxas
Xiaolei Huang
Debabrata Banerjee
Keywords:
Image analysis
Tissue Imaging