Schematic summary of the dNANOSIS assay
Point-of-Care (POC) testing is defined as a simple operated, easy-to-read, rapid, and low-cost medical diagnosis that can be used by non-specialists at home, in the field or in an ambulance. It provides timely diagnostic results, enabling early diagnosis of diseases, protective therapy and rapid treatment of the patients. POC testing requires unique features, including: (1) simplified operation (e.g. one-pot assay) by non-specialists at diverse locations, (2) easy-to-read signals, (3) a reliable and rapid assay and (4) low cost.
Rutgers inventors developed DNA nanodevices that can be adopted for POC testing. The nanodevices contain a limited number of oligonucleotides and a simple catalytic system. Target molecules can be identified by visible color change. It does not require any additional steps to separate detectable components from the assay background and can be applied to in-home use or diagnoses at diverse locations. As proof-of-concept, Dr. Fu's team has developed and tested DNA nanodevices to detect a microRNA biomarker for prostate cancer at concentrations as low as 0.1 nM.
Similar nucleic acid nanodevices can be readily designed for the detection of other disease-relevant nucleic acids such as SARS-COV-2 RNA and small molecules. This technology can be readily extended to a low-cost, rapid and portable system.
- One-pot assay with color change
- One-step detection of target molecules
- Low cost
- POC testing for a variety of disease-relevant nucleic acids, small molecules and proteins, on a rapid and low-cost detection platform with visible color changes
- POC testing for cancer diagnosis by detecting circulating miRNAs or single-nucleotide variants (SNVs)
- POC testing to monitor drug food safety and metal pollution
Intellectual Property & Development Status:
US Patent 62/647,014; US 10,961,565 B2. Available for licensing and/or search collaboration. Please contact email@example.com.
Sung Won Oh, Adriana Pereira, Ting Zhang and Jinglin Fu DNA-Mediated Proximity Assembly Circuit for Actuating Biochemical Reactions, Angewandte Chemie 2018, 57, 13086-13090.
Santiago-McRae, E., et al. (2022) Rapid Nucleic Acid Reaction Circuits for Point-of-care Diagnosis of Diseases. Curr Top Med Chem. 22(8): 686-698.