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SuperSelective PCR Primers
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Structure of a SuperSelective primer for the detection and quantitation of rare mutant sequences in the presence of 1,000,000 wild-type sequences; and a demonstration that the number of amplification cycles required to generate a signal above background is inversely linearly proportional to the logarithm of the number of mutant molecules in the original...
Published: 10/9/2024
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Inventor(s): Salvatore Marras, Diana Vargas-Gold, Sanjay Tyagi, Fred Kramer
Keywords(s): Anti-cancer, Sensors & Probes
Category(s): Technology Classifications > Healthcare & Life Sciences, Technology Classifications > Research Tools, Technology Classifications > Diagnostics
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High-throughput gene expression screening in activated T-cells using FISH-Flow
Induction of gene expression and subsequent detection by FISH-Flow. c-fos expression is induced in activated cells, which are readily identified by FISH-Flow. GFP: negative control, GAPDH: positive control.
Invention Summary:
Many immune responses to pathological conditions involve the activation of T-cells by specific antigens. The interactions...
Published: 12/4/2024
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Inventor(s): Yuri Bushkin, Maria Gennaro, Sanjay Tyagi, Richard Pine
Keywords(s): Sensors & Probes
Category(s): Technology Classifications > Healthcare & Life Sciences, Technology Classifications > Diagnostics
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High-fidelity amplified FISH (amp-FISH) for sensitive and specific in situ detection of RNA
amp-FISH probes can distinguish single-nucleotide variations within single mRNA molecules and produces amplified signals in situ for both mutant and wild-type variants, each in a distinct color.
Invention Summary:
Fluorescence in situ hybridization (FISH) is used in clinical and research applications to detect and visualize specific nucleic acid...
Published: 12/4/2024
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Inventor(s): Sanjay Tyagi, Salvatore Marras
Keywords(s): Sensors & Probes
Category(s): Technology Classifications > Healthcare & Life Sciences, Technology Classifications > Research Tools, Technology Classifications > Diagnostics
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